There currently is one option to travel by night train from Vienna to La Spezia, taking between approximately 16 and 17 hours: Namely the NJ 233 operated by ÖBB.
The Austrian Federal Railways (ÖBB) operate the Nightjet and Euronight trains (the latter together with other national railway agencies). The ÖBB usually offer three types of carriages in their night trains: seats, couchettes, and sleepers. While seats are only for the bravest, couchettes and sleepers offer a more comfortable journey. Couchettes accommodate up to six people, while sleepers are private compartments for one to three people. They sometimes include a private bathroom or even a shower, sometimes only a sink.
For the next 7 dates with connections, the cheapest offer starts at 35 EUR. Check our connection pages below for all availabilities!