There currently is one option to travel by night train from Pardubice to Vienna, taking approximately 5 hours: Namely the NJ 457 operated by ÖBB.
The Austrian Federal Railways (ÖBB) operate the Nightjet and Euronight trains (the latter together with other national railway agencies). ÖBB night trains typically feature three types of carriages: seats, couchettes, and sleepers. Seats are for the bravest, while couchettes and sleepers provide a more comfortable journey. Couchettes accommodate up to six people, while sleepers are private compartments for one to three people. They sometimes include a private bathroom or even a shower, sometimes only a sink.
For the next 7 dates with connections, the cheapest offer starts at 35 EUR. Check our connection pages below for all availabilities!