There currently is one option to travel by night train from Prague (Czech Republic) to Hamburg (Germany), taking between approximately 6 and 7 hours: Namely the RJ 478. These lines are operated by České dráhy and Deutsche Bahn.
ČD, the Czech national railway, operates domestic and cross-border passenger trains linking the Czech Republic with its neighbors.
Deutsche Bahn (DB) exited the actual night train business in 2016. However, it operates around a dozen Intercity and Eurocity trains that run exclusively at night.
In these night trains, there are only first and second class seats. The lights stay on all the time and there is no way to recline the seats.
For the next 7 dates with connections, the cheapest offer starts at 125 EUR. Check our connection pages below for all availabilities!