There currently is one option to travel by night train from Hamburg (Germany) to Prague (Czech Republic), taking between approximately 6 and 7 hours: Namely the RJ 479. These lines are operated by Deutsche Bahn and České dráhy.
Deutsche Bahn (DB) exited the actual night train business in 2016. However, it operates around a dozen Intercity and Eurocity trains that run exclusively at night.
In these night trains, there are only first and second class seats. The lights stay on all the time and there is no way to recline the seats.
České dráhy (ČD) is the Czech national railway operator, running domestic and international passenger trains connecting the Czech Republic with neighboring countries.
For the next 7 dates with connections, the cheapest offer starts at 77 EUR. Check our connection pages below for all availabilities!